Lambda Protein Phosphatase (Lambda PP)
Sizes: 20KU, 100KU
Catalogue Numbers: VK401012-20, VK401012-100
Citations, Manuals and MSDS Available upon request.
Description: Lambda Protein Phosphatase (Lambda PP) is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues. One unit is defined as the amount of enzyme that hydrolyzes 1 nmol of p-Nitrophenyl Phosphate (50 mM) in 1 minute at 30°C in a total reaction volume of 50 µl.
Form: Liquid
Storage buffer: 100 mM NaCl, 50 mM HEPES, pH 7.5, 0.1 mM MnCl2, 0.1 mM EDTA, 2 mM DTT, 0.01% Brij35, and 50% glycerol.
Purity: >90% as determined by SDS-PAGE.
Applications: Remove phosphate groups from phosphorylated serine, threonine and tyrosine residues in proteins.
Expression system: E. coli
Protein length: Lambda PP is cloned from Escherichia phage lambda and expressed in E. coli.
Nature: Recombinant
Predicted molecular weight: 27.32 kDa
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week .Store at -20 to -80°C for twelve months from the date of receipt.
Species: Escherichia phage lambda (Bacteriophage lambda)
Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Avoid repeated freeze/thaw cycles. For research use only.