Product Description

LATS1/2 (phospho-Ser909) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES15191-50, ES15191-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatmen

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, ELISA, IHC

Alternate Names: Serine/threonine-protein kinase LATS1 (EC 2.7.11.1) (Large tumor suppressor homolog 1) (WARTS protein kinase) (h-warts)

Clonality: Polyclonal

Immunogen: Synthesized phosho peptide around human LATS1/2 (Ser909)

Isotype: IgG

Target: LATS1/2

Specificity: This antibody detects endogenous levels of Human LATS1/2 (phospho-Ser909)

Dilution: WB 1:500-2000, IHC-p 1:50-300, ELISA 2000-20000

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in all adult tissues examined except for lung and kidney,

Observed Band (KD): 140kD

Fields: Hippo signaling pathway, Hippo signaling pathway - multiple species

Function: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein
Cofactor: Magnesium
Function: Tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDC2 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function
PTM: Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3 at Ser-909 and Thr-1079, which results in its activation. Phosphorylated upon DNA damage, probably by ATM or ATR
Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family
Similarity: Contains 1 AGC-kinase C-terminal domain
Similarity: Contains 1 protein kinase domain

Subcellular Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle Midbody Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis

Storage and Stability: -20°C/1 year

Gene Name: LATS1 WARTS

Protein Name: Serine/threonine-protein kinase LATS1 (EC 2.7.11.1) (Large tumor suppressor homolog 1) (WARTS protein kinase) (h-warts)

Mouse Gene ID: 16798

Mouse SwissProt Number: Q8BYR2

Research Use Only