Product Description

Lck (phospho Ser540) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES6093-50, ES6093-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Aug 2016],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: LCK, Tyrosine-protein kinase Lck, Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, Protein YT16, Proto-oncogene Lck, T cell-specific protein-tyrosine kinase, p56-LCK

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human LCK around the phosphorylation site of Ser59. AA range:26-75

Isotype: IgG

Target: Lck

Specificity: Phospho-Lck (S540) Polyclonal Antibody detects endogenous levels of Lck protein only when phosphorylated at S540.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed specifically in lymphoid cells,

Observed Band (KD): 58kD

Fields: NF-kappa B signaling pathway, Osteoclast differentiation, Natural killer cell mediated cytotoxicity, Th1 and Th2 cell differentiation, Th17 cell differentiation, T cell receptor signaling pathway, Yersinia infection, Human T-cell leukemia virus 1 infection, PD-L1 expression and PD-1 checkpoint pathway in cancer, Primary immunodeficiency

Function: Catalytic Activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Disease: A chromosomal aberration involving LCK is found in leukemias. Translocation t(1, 7)(p34, q34) with TCRB
Domain: The SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation
Enzyme Regulation: Inhibited by tyrosine phosphorylation
Function: Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associat

Subcellular Location: Cell membrane, Lipid-anchor, Cytoplasmic side Cytoplasm, cytosol Present in lipid rafts in an inactive form

Storage and Stability: -20°C/1 year

Gene Name: LCK

Protein Name: Tyrosine-protein kinase Lck

Mouse Gene ID: 16818

Mouse SwissProt Number: P06240

Rat Gene ID: 313050

Rat SwissProt Number: Q01621

Research Use Only