LI-cadherin (T363) Polyclonal Antibody-BS2396

LI-cadherin (T363) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2396-50, BS2396-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q12864

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:200

Background: The cadherins are a family of Ca++-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including catenin β, to regulate cadherin function. LI-cadherin (for liver-intestine-cadherin) expression is restricted to liver and intestine tissues and is specifically localized to the basolateral domain of hepatocytes and enterocytes.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: LI-cadherin (T363) polyclonal antibody detects endogenous levels of LI-cadherin protein.

Bioworld Molecular Weight: ~90 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of LI-cadherin (T363) Antibody at 1:2000 dilution
Lane 1: SGC7901 whole cell lysate (40ug)
Lane 2: HCT116 whole cell lysate (40ug)
Lane 3: CT26 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of LI-cadherin (T363) pAb in paraffin-embedded human colon carcinoma tissue at 1:5000. cell membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of LI-cadherin (T363) pAb in paraffin-embedded human rectum carcinoma tissue at 1:5000. cell membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: CDH17; Cadherin-17;

Immunogen: Synthetic peptide, corresponding to amino acids 350-400 of Human LI-cadherin.

Conjugate: Unconjugated

Modification: Unmodified