LIMK1 Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-93157-60, E-AB-93157-120, E-AB-93157-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: LIMK1
Target Synonym: LIMK1; LIMK; LIMK-1
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Neuroscience, Signal Transduction
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: P53667
Background: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Concentration: 1mg/mL
Immunogen: Recombinant fusion protein of human LIMK1
Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000
Calculated MW: 33kDa/68kDa/70kDa/72kDa
Observed MW: 73kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only