LRG1 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS70957-50, BS70957-100 Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: P02750 Host: Rabbit Reactivity: Human, Mouse, Rat Applications: WB, IF/ICC All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 Background: The leucine-rich repeat (LRR) family of proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation (O'Donnell et al., 2002 [PubMed 12223515]). Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Polyclonal Antibodies Bioworld Molecular Weight: 45KDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of extracts of Human plasma, using LRG1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per Lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s., Immunofluorescence analysis of rat liver cells using LRG1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of human liver cells using LRG1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of mouse liver cells using LRG1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining. Alternative Name: LRG1; HMFT1766; LRG Immunogen: Recombinant fusion protein of human LRG1(NP_443204.1). Conjugate: Unconjugated Modification: Unmodified