Product Description

LRPPRC Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS70542-50, BS70542-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P42704

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:1000 - 1:2000 | IHC,1:100 - 1:200 | IF/ICC,1:100 - 1:200

Background: This gene encodes a leucine-rich protein that has multiple pentatricopeptide repeats (PPR). The precise role of this protein is unknown but studies suggest it may play a role in cytoskeletal organization, vesicular transport, or in transcriptional regulation of both nuclear and mitochondrial genes. The protein localizes primarily to mitochondria and is predicted to have an N-terminal mitochondrial targeting sequence. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 130kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using LRPPRC antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s., Immunohistochemistry of paraffin-embedded human breast cancer using LRPPRC Rabbit pAb at dilution of 1:250. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human colon carcinoma using LRPPRC Rabbit pAb at dilution of 1:250. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Confocal immunofluorescence analysis of Hela cells using LRPPRC Polyclonal Antibody at dilution of 1:400. Blue: DAPI for nuclear staining.

Alternative Name: LRPPRC; CLONE-23970; GP130; LRP130; LSFC

Immunogen: Recombinant fusion protein of human LRPPRC(NP_573566.2).

Conjugate: Unconjugated

Modification: Unmodified