Product Description

M-RIP Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: CAS7516-50, CAS7516-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q6WCQ1

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP, IHC

All Applications: WB: 1:5000~1:10000
IP:1:2000~1:8000
IHC:1:50~1:100

Background: Myosin phosphatase-rho interacting protein (M-RIP), also known as p116RIP, RIP3, and MPRIP, localizes to actin-myosin filaments regulating cytoskeletal dynamics. M-RIP contains amino-terminal pleckstrin homology domains, carboxyl-terminal coiled-coil domains, and was originally identified to associate with the myosin phosphatase complex. M-RIP binds to MBS/MYRT, the myosin binding subunit of myosin phosphatase, as well as RhoA. Phosphorylation of MYRT by Rho-associated kinase (ROCK) inhibits myosin phosphatase activity, resulting in increased levels of phosphorylation on myosin light chain, and enhanced contractility. M-RIP may function as a scaffolding protein for the complex between the myosin phosphatase complex, Rho/ROCK, and actin. Silencing of M-RIP results in disassembly of the complex, increased phosphorylation of myosin light chain, and changes to cytoskeletal dynamics.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: M-RIP polyclonal antibody detects endogenous levels of M-RIP protein.

Bioworld Molecular Weight: ~ 130-160kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of M-RIP polyclonal antibody at 1:5000 dilution
Lane 1: PC12 whole cell lysate (40ug)
Lane 2: CT-26 whole cell lysate (40ug)
Lane 3: H1792 whole cell lysate (40ug)
Lane 4: Myla2059 whole cell lysate (40ug)
Lane 5: PC3 whole cell lysate (40ug)
Lane 6: SGC7901 whole cell lysate (40ug), Immunoprecipitation - M-RIP Polyclonal Antibody, Immunohistochemistry of paraffin-embedded human colon carcinoma using M-RIP antibody at dilution of 1:50., Immunohistochemistry of paraffin-embedded human colon carcinoma using M-RIP antibody at dilution of 1:50.

Alternative Name: Myosin phosphatase Rho-interacting protein;
M-RIP; Rho-interacting protein 3; RIP3; p116Rip; MPRIP

Immunogen: Recombinant protein of human M-RIP.

Conjugate: Unconjugated

Modification: Unmodified