Product Description

MAFA Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES15057-50, ES15057-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS, MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).[supplied by OMIM, Mar 2008],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB

Alternate Names: Transcription factor MafA (Pancreatic beta-cell-specific transcriptional activator) (Transcription factor RIPE3b1) (V-maf musculoaponeurotic fibrosarcoma oncogene homolog A)

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human MAFA AA range: 204-254

Isotype: IgG

Target: MAFA

Specificity: This antibody detects endogenous levels of MAFA at Human/Mouse

Dilution: WB 1:500-2000

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.36% sodium azide.

Expression: Expressed in the islets of Langerhans (at protein level),

Observed Band (KD): 38kD

Fields: Type II diabetes mellitus, Maturity onset diabetes of the young

Function: Function: Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context
Induction: Up-regulated by glucose
PTM: Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation

Subcellular Location: Nucleus

Storage and Stability: -20°C/1 year

Gene Name: MAFA

Protein Name: MAFA

Mouse Gene ID: 378435

Mouse SwissProt Number: Q8CF90

Research Use Only