Product Description

MDM2 (Phospho-S186/S188) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS67430-50, BS67430-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: MDM2, a ubiquitin ligase for p53, plays a central role in regμlation of the stability of p53 . Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53.

Category: Primary Antibody

Reactivity: Human, Dog

Host: Rabbit

Applications: WB, IF/ICC

Alternate Names: E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MDM2 with a site at pS186/S188. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/200)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of MDM2 with a site at pS186/S188 protein.

Molecular Weight: ~ 105 kDa

Pathway: ErbB HER Signaling，Cell Cycle G2 M DNA Damage Signaling Pathway，PI3K AKT signaling.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q00987

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only