Product Description

MDM2 (phospho Ser186/S188) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS65101-50, BS65101-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: MDM2 proto-oncogene(MDM2) Homo sapiens This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regμlated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing resμlts in a mμltitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013],

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: IF/ICC, WB, ELISA

Alternate Names: MDM2, E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: The antiserum was produced against synthesized peptide derived from human MDM2 around the phosphorylation site of Ser186 and Ser188. AA range:151-200

Dilution: WB 1:500-2000 Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity: Phospho-MDM2 (S186/S188) Polyclonal Antibody detects endogenous levels of MDM2 protein only when phosphorylated at S186/S188.

Molecular Weight: /

Pathway: Examples of Crosstalk Between Post-translational Modifications,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q00987

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Research Use Only