Product Description

Med8 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4634-50, ES4634-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: MED8, Mediator of RNA polymerase II transcription subunit 8, Activator-recruited cofactor 32 kDa component, ARC32, Mediator complex subunit 8

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from Med8. at AA range: 70-150

Isotype: IgG

Target: Med8

Specificity: Med8 Polyclonal Antibody detects endogenous levels of Med8 protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Cervix carcinoma, Melanoma, Testis,

Observed Band (KD): 29kD

Function: Domain: The elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV]
Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins
Pathway: Protein modification, protein ubiquitination
Similarity: Belongs to the Mediator

Subcellular Location: Nucleus

Storage and Stability: -20°C/1 year

Gene Name: MED8

Protein Name: Mediator of RNA polymerase II transcription subunit 8

Mouse Gene ID: 80509

Mouse SwissProt Number: Q9D7W5

Research Use Only