Product Description

Met (phospho Tyr1234) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1360-50, ES1360-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016],

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Applications: WB, ELISA, IHC

Alternate Names: MET, Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Met around the phosphorylation site of Tyr1234. AA range:1201-1250

Isotype: IgG

Target: Met

Specificity: Phospho-Met (Y1234) Polyclonal Antibody detects endogenous levels of Met protein only when phosphorylated at Y1234.

Dilution: WB 1:500-2000, IHC-p 1:50-300, ELISA 2000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine, Found also in basal keratinocytes of esophagus and skin, High levels are found in liver, gastrointestinal tract, thyroid and kidney, Also present in the brain, Expressed in metaphyseal bone (at protein level) (PubMed:26637977),

Observed Band (KD): 145kD

Fields: EGFR tyrosine kinase inhibitor resistance, MAPK signaling pathway, Ras signaling pathway, Rap1 signaling pathway, Calcium signaling pathway, PI3K-Akt signaling pathway, Axon guidance, Focal adhesion, Adherens junction, Bacterial invasion of epithelial cells, Epithelial cell signaling in Helicobacter pylori infection, Malaria, Pathways in cancer, Transcriptional misregulation in cancer, Proteoglycans in cancer, MicroRNAs in cancer, Chemical carcinogenesis - reactive oxygen species, Renal cell carcinoma, Melanoma, Non-small cell lung cancer, Hepatocellular carcinoma, Gastric cancer, Central carbon metabolism in cancer

Function: Catalytic Activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Disease: Activation of MET after rearrangement with the TPR gene produces an oncogenic protein
Disease: Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM: 114550]
Disease: Defects in MET are a cause of hereditary papillary renal carcinoma (HPRC) [MIM: 605074], also known as papillary renal cell carcinoma 2 (RCCP2). HPRC is a form of inherited kidney cancer characterized by a predisposition to develop multiple, bilateral papillary renal tumors. The pattern of inheritance is consistent with autosomal dominant transmission with reduced penetrance
Disease: Defects in MET may be associated with gastric cancer
Disease: Genetic variations in MET may be associated with susceptibility to autism type 9 (AUTS9) [MIM: 611015]. Autism is a neurodevelopmental disorder characterized by disturbance in l

Subcellular Location: Membrane, Single-pass type I membrane protein, [Isoform 3]: Secreted

Storage and Stability: -20°C/1 year

Gene Name: MET

Protein Name: Hepatocyte growth factor receptor

Mouse SwissProt Number: P16056

Rat Gene ID: 24553

Rat SwissProt Number: P97523

Research Use Only