Product Description

Mitochondrial Complex Ⅲ (Coenzyme Q-Cytochrome C Reductase) Activity Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K151-M-48, E-BC-K151-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: As an organelle, mitochondria is the "power factory" in cells and the main site of aerobic respiration of cells. Its function is to convert energy through oxidative phosphorylation to provide energy for cellular activities. The oxidation process is carried out by four respiratory chain membrane protein complexes (complexes I, II, III and IV) on the inner mitochondrial membrane. mitochondrial complex III, also known as cytochrome c reductase complex, its main function is to oxidize the reduced coenzyme Q10 formed by mitochondrial complexes I and II to oxidative coenzyme Q10. In this process, the OD value increased at 550 nm. Therefore, the activity of mitochondrial complex III can be quantified by measure the change OD value at 550 nm.

Research Areas: Mitochondria Function;cuproptosis;Energy Metabolism

Result Type: Enzyme activity

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 4.45-106.8 U/L

Tested Sample Types: Animal tissue

Assay Length: 30 min

Sensitivity: 4.45 U/L

Inter cv: 10%

Intra cv: 5%

Research Use Only