Product Description

MKK3 (Phospho-T222) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS64557-50, BS64557-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinaseegμlatory threonine and tyrosine residues present in thehr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6hosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

Category: Primary Antibody

Reactivity: Human, Mouse, Zebrafish

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, MEK 3, Stress-activated protein kinase kinase 2, SAPK kinase 2, SAPKK-2, SAPKK2, MAP2K3, MEK3, MKK3, PRKMK3, SKK2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human MKK3 around the phosphorylation site of Threonine 222.

Dilution: WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: MKK3 (Phospho-T222) polyclonal antibody detects endogenous levels of MKK3 protein only when phosphorylated at Thr222.

Molecular Weight: ~ 40 kDa

Pathway: Signaling Pathways Activating p38 MAP Kinase,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P46734

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only