MLF1/MLF2 Polyclonal Antibody-BS65449

MLF1/MLF2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65449-50, BS65449-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: Q71F23

Host: Rabbit

Reactivity: Human, Mouse, Rat, Pig, Cow, Sheep,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MLF1/MLF2 Polyclonal Antibody detects endogenous levels of MLF1/MLF2 protein.

Bioworld Molecular Weight: 31kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MLF1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C., Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MLF1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C., Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MLF1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.

Alternative Name: Hls7; MLF1; MLF2; MLF3; MLF1_HUMAN; Myelodysplasia myeloid leukemia factor 1; Myelodysplasia-myeloid leukemia factor 1; Myeloid leukemia factor 1; myeloid leukemia factor 1 variant 1; myeloid leukemia factor 1 variant 2; myeloid leukemia factor 1 variant 3.

Immunogen: KLH conjugated synthetic peptide derived from human MLF1/2/3:165-268/268

Conjugate: Unconjugated

Modification: Unmodified