MLH1 Monoclonal Antibody - BT-MCA4592

MLH1 Monoclonal Antibody

Sizes: 50μL, 100μL

Catalogue Number: BT-MCA4592-50, BT-MCA4592-100

Citations, Manuals and MSDS Available upon request.

Background: DNA-mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. Lack of a functional DNA-mismatch repair pathway is a common characteristic of several different types of human cancers, either due to an MMR gene mutation or promoter-methylation gene silencing. MLH1 is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in hereditary nonpolyposis colon cancer (HNPCC). MLH1 is an integral part of the protein complex responsible for mismatch repair expressed in lymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid and gall bladder, and is methylated in several ovarian tumors. Loss of MLH1 protein expression is associated with a mutated phenotype, microsatellite instability and a predisposition to cancer. In hereditary nonpolyposis colorectal cancer (HNPCC), an autosomal dominant inherited cancer syndrome that signifies a high risk of colorectal and various other types of cancer, the MLH1 gene exhibits a pathogenic mutation. Inactivation of the MLH1 gene causes genome instability and predisposition to cancer. MLH1 also plays a role in meiotic recombination.

Research Areas: Epigenetics and Nuclear Signaling

Target Protein: MLH1

Applications: WB, IHC, ICC

Reactivity: Human, Monkey

Clonality: Monoclonal

Clone ID: 4C9C7

Host: Mouse

Isotype: Mouse IgG1

Full Product Name: MLH1

Immunogen: Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli.

Storage: 4°C|-20°C for long term storage

Formulation: Ascitic fluid containing 0.03% sodium azide.

Molecular Weight (Da): 85kDa

UniProt Accession: P40692

Synonyms: FCC2, COCA2, HNPCC

GeneID: 4292

Research Use Only