MonoMethyl-Histone H3 (Lys4) Polyclonal Antibody-BZ17063

MonoMethyl-Histone H3 (Lys4) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ17063-50, BZ17063-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: Q16695

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, IP, , ChIP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 ChIP: 1/20

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 16 kDa; Observed MW: 16 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MonoMethyl-Histone H3 in various cell lines lysates using MonoMethyl-Histone H3-K4 antibody., MonoMethyl-Histone H3-K4 antibody Dotblot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K4 antibody., Immunohistochemistry analysis of paraffin-embedded rat spleen using MonoMethyl-Histone H3-K4 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using MonoMethyl-Histone H3-K4 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: H3K4me; H3 histone; HIST1H3A; Histone cluster 1; H3a

Immunogen: A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3

Conjugate: Unconjugated

Modification: Methylated