Mouse 6ketoPGF1α (6-keto-PGF1 alpha) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK11243-48, ELK11243-96, ELK11243-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 6-keto-PGF1 alpha; 6ketoPGF1α
Assay Type: Competitive Inhibition
Sensitivity: 2.4 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h
Research Areas: Neuroscience
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse 6ketoPGF1α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 6ketoPGF1α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 6ketoPGF1α in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only