Product Description

Mouse AIF (Apoptosis Inducing Factor) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK8448-48, ELK8448-96, ELK8448-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse AIF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AIF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse AIF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AIF in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: PDCD8, Programmed Cell Death protein 8, Apoptosis-inducing factor 1, mitochondrial

Reactivity: Mouse

Sample Type: Serum, Plasma, Tissue Homogenates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Signal Transduction, Apoptosis

Assay Length: 3.5h

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

UniProt: Q9Z0X1

Research Use Only