Product Description

Mouse IL-27 (Interleukin 27) Microsample ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK9612MS-48, ELK9612MS-96, ELK9612MS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-27. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL-27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL-27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL-27 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: IL-27, IL-27A, IL27p28, IL30, MGC71873, p28,

Reactivity: Mouse

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Immunology, Cancer

Assay Length: 3.5h

Sensitivity: 19.12 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

UniProt: Q8K3I6

Research Use Only