Mouse IL-27 (Interleukin 27) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK9612MS-48, ELK9612MS-96, ELK9612MS-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: IL-27; IL-27A; IL27p28; IL30; MGC71873; p28;
Assay Type: Sandwich
Sensitivity: 19.12 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Areas: Immunology; Cancer
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-27. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL-27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL-27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL-27 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q8K3I6
Research Use Only