Mouse IRF3 (Interferon Regulatory Factor 3) ELISA Kit - ELK2854

Mouse IRF3 (Interferon Regulatory Factor 3) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK2854-48, ELK2854-96, ELK2854-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Irf3; IRF-3

Assay Type: Sandwich

Sensitivity: 0.055 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Length: 3.5h

Research Areas: Infection immunity; Immune molecule;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IRF3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IRF3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IRF3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IRF3 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P70671

Research Use Only