Product Description

Mouse NFκB-p65 (Nuclear Factor Kappa B p65) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK8449-48, ELK8449-96, ELK8449-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: NFKB3, p65, REL-A, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3, Transcription Factor P65, Nuclear factor NF-kappa-B p65 subunit, RELA, V-Rel Reticuloendotheliosis Viral Oncogene Homolog A

Reactivity: Mouse

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Apoptosis

Assay Length: 3.5h

Sensitivity: 11.5 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

UniProt: Q04207

Research Use Only