Mouse NUCB2 (Nucleobindin 2) ELISA Kit - ELK9838

Mouse NUCB2 (Nucleobindin 2) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9838-48, ELK9838-96, ELK9838-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: NUCB2; NEFA; Nucleobindin 2; DNA-binding protein NEFA; Gastric cancer antigen Zg4; Prepronesfatin

Assay Type: Sandwich

Sensitivity: 0.1 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Length: 3.5h

Research Areas: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NUCB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NUCB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NUCB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NUCB2 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P81117

Research Use Only