Product Description

Mouse PDGFRb (Platelet Derived Growth Factor Receptor Beta) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11975-48, ELK11975-96, ELK11975-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Mouse

Alternate Names: PDGF-R-beta, PDGFR-beta,Beta platelet-derived growth factor receptor,Beta-type platelet-derived growth factor receptor,CD140 antigen-like family member B,CD140b,PDGFR-1,

Sensitivity: 0.113 ng/mL

Assay Type: Sandwich

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Signal Transduction, Tumor Immunity, Infection Immunity, Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PDGFRb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PDGFRb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PDGFRb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PDGFRb in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P05622

Research Use Only