Mouse PIICP (Procollagen II C-Terminal Propeptide) ELISA Kit - ELK5532

Mouse PIICP (Procollagen II C-Terminal Propeptide) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK5532-48, ELK5532-96, ELK5532-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: P2CP; C-Propeptide Of Type II Procollagen; Procollagen II Carboxy Terminal Propeptide

Assay Type: Competitive Inhibition

Sensitivity: 1.45 ng/mL

Standard: 300 ng/mL

Detection Range: 4.69-300 ng/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Infection immunity; Bone metabolism; Rheumatology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse PIICP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIICP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIICP in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only