Product Description

MRLC2 (phospho Ser20) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES5880-50, ES5880-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: MYL9, MLC2, MRLC1, MYRL2, Myosin regulatory light polypeptide 9, 20 kDa myosin light chain, LC20, MLC-2C, Myosin RLC, Myosin regulatory light chain 2, smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRL

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Myosin regulatory light chain 2 around the phosphorylation site of Ser18. AA range:3-52

Isotype: IgG

Target: Myosin Light Chain 2

Specificity: Phospho-MRLC2 (S20) Polyclonal Antibody detects endogenous levels of MRLC2 protein only when phosphorylated at S20.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Smooth muscle tissues and in some, but not all, nonmuscle cells,

Observed Band (KD): 18kD

Fields: cGMP-PKG signaling pathway, cAMP signaling pathway, Vascular smooth muscle contraction, Axon guidance, Focal adhesion, Tight junction, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Oxytocin signaling pathway, Shigellosis, Salmonella infection

Function: Function: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion
Miscellaneous: This chain binds calcium
PTM: Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge
Similarity: Contains 3 EF-hand domains
Subunit: Myosin is an hexamer of 2 heavy chains and 4 light chains
Tissue Specificity: Smooth muscle tissues and in some, but not all, nonmuscle cells

Subcellular Location: Cytoplasm, cytoskeleton Cytoplasm, cell cortex Colocalizes with F-actin, MYH9 and PIEZO1 at the actomyosin cortex in myoblasts

Storage and Stability: -20°C/1 year

Gene Name: MYL9

Protein Name: Myosin regulatory light polypeptide 9

Mouse Gene ID: 98932

Mouse SwissProt Number: Q9CQ19

Rat Gene ID: 296313

Rat SwissProt Number: Q64122

Research Use Only