MRPS7 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS77120-50, BS77120-100 Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: Q9Y2R9 Host: Rabbit Reactivity: Human, Mouse, Rat Applications: WB, IF/ICC All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. In the prokaryotic ribosome, the comparable protein is thought to play an essential role in organizing the 3' domain of the 16 S rRNA in the vicinity of the P- and A-sites. Pseudogenes corresponding to this gene are found on chromosomes 8p and 12p. Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Unmodification Bioworld Molecular Weight: 28kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of extracts of various cell lines, using MRPS7 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per Lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s., Immunofluorescence analysis of C6 cells using MRPS7 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of L929 cells using MRPS7 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of U-2 OS cells using MRPS7 antibody at dilution of 1:100. Blue: DAPI for nuclear staining. Alternative Name: MRPS7; MRP-S; MRP-S7; RP-S7; RPMS7; S7mt; bMRP27a Immunogen: Recombinant fusion protein of human MRPS7(NP_057055.2). Conjugate: Unconjugated Modification: Unmodified