MTHFD1L Polyclonal Antibody-BS71022

MTHFD1L Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS71022-50, BS71022-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q6UB35

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200

Background: The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 106kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using MTHFD1L antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunohistochemistry of paraffin-embedded rat brain using MTHFD1L Rabbit pAb at dilution of 1:200. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse testis using MTHFD1L Rabbit pAb at dilution of 1:200. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse spinal cord using MTHFD1L Rabbit pAb at dilution of 1:200. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Alternative Name: MTHFD1L; FTHFSDC1; MTC1THFS; dJ292B18.2

Immunogen: Recombinant fusion protein of human MTHFD1L(NP_056255.2).

Conjugate: Unconjugated

Modification: Unmodified