Product Description

Myeloperoxidase Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66214-50, MB66214-100

Product: Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Swiss-Prot: P05164

Host: Mouse

Reactivity: Human

Applications: IHC

All Applications: IHC (1/100 - 1/300)

Background: Myeloperoxidase (MPO) is a peroxidase enzyme that is part of the host defense system of polymorphonuclear leukocytes (reviewed in 1). The gene for MPO was cloned independently from several laboratories (2-5). A decrease in MPO expression was noticed upon differentiation of HL-60 cells . MPO catalyzes the reaction of hydrogen peroxide and chloride (or other halides) to produce hypochlorous acid and other potent antimicrobial oxidants. Knockout mice of MPO are impaired in clearing select microbial infections . Processing of mature MPO from an initial 80-90 kDa translation product involves insertion of a heme moiety, glycosylation, and proteolytic cleavage. The mature protein is a tetramer of two heavy chains (60 kDa) and two light chains (12 kDa). It is abundantly expressed in neutrophils and monocytes and secreted during their activation. Heightened MPO levels have been associated with tissue damage and a number of pathological conditions

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Myeloperoxidase protein.

Bioworld Molecular Weight: ~ 83 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemical analysis of Myeloperoxidase staining in human spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunohistochemical analysis of Myeloperoxidase staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Alternative Name: Myeloperoxidase; MPO

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Myeloperoxidase. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified