Product Description

MYL9 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7173-50, BS7173-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P24844

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 18KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using MYL9 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunofluorescence analysis of Rat intestine using MYL9 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of Human colon carcinoma using MYL9 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of Mouse intestine using MYL9 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: MYL9; LC20; MLC-2C; MLC2; MRLC1; MYRL2

Immunogen: A synthetic peptide of human MYL9(NP_006088.2).

Conjugate: Unconjugated

Modification: Unmodified