Product Description

MYOF Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES14598-50, ES14598-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Mutations in dysferlin, a protein associated with the plasma membrane, can cause muscle weakness that affects both proximal and distal muscles. The protein encoded by this gene is a type II membrane protein that is structurally similar to dysferlin. It is a member of the ferlin family and associates with both plasma and nuclear membranes. The protein contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. Two transcript variants encoding different isoforms have been found for this gene. Other possible variants have been detected, but their full-length nature has not been determined. [provided by RefSeq, Dec 2008],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human MYOF AA range: 868-918

Isotype: IgG

Target: MYOF

Specificity: This antibody detects endogenous levels of MYOF at Human/Mouse

Dilution: WB 1：500-2000

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in myoblast and endothelial cells (at protein level), Highly expressed in cardiac and skeletal muscles, Also present in lung, and at very low levels in kidney, placenta and brain,

Molecular Weight (Da): 227kD

Function: Cofactor: Binds calcium ions. The ions are bound to the C2 1 domain
Domain: The C2 domain 1 associates with lipid membranes in a calcium-dependent manner
Function: Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR
Similarity: Belongs to the ferlin family
Similarity: Contains 5 C2 domains
Subcellular Location: Concentrated at the membrane sites of both myoblast-myoblast and myoblast-myotube fusions. Detected at the plasmalemma in endothelial cells lining intact blood vessels (By similarity). Found at nuclear and plasma membranes. Enriched in undifferentiated myoblasts near the plasma membrane in puncate structures
Subunit: Interacts with DNM

Subcellular Location: Cell membrane, Single-pass type II membrane protein Nucleus membrane, Single-pass type II membrane protein Cytoplasmic vesicle membrane, Single-pass type II membrane protein Concentrated at the membrane sites of both myoblast-myoblast and myoblast-myotube fusions Detected at the plasmalemma in endothelial cells lining intact blood vessels (By similarity) Found at nuclear and plasma membranes Enriched in undifferentiated myoblasts near the plasma membrane in puncate structures

Storage and Stability: -20°C/1 year

Gene Name: MYOF FER1L3 KIAA1207

Protein Name: MYOF

Mouse Gene ID: 226101

Mouse SwissProt Number: Q69ZN7

Research Use Only