NARF Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-18713-60, E-AB-18713-120, E-AB-18713-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: NARF
Target Synonym: DKFZp434G0420; FLJ10067; GC17P078009; IOP2; Iron only hydrogenase like protein 2; Nuclear prelamin A recognition factor; Prenyl dependent prelamin A binding protein
Research Areas: Epigenetics and Nuclear Signaling, Neuroscience, Signal transduction
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: IHC, ELISA
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: Q9UHQ1
Background: Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.
Concentration: 1.56 mg/mL
Immunogen: Fusion protein of human NARF
Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Purification Method: Antigen affinity purification
Dilution: IHC 1:50-1:300, ELISA 1:5000-1:10000
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only