Product Description

Nck2 (I364) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1455-50, BS1455-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O43639

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

All Applications: IHC: 1:50~1:200

Background: The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 (NCKα) and NCK2 (NCKβ), which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. Specifically, overexpression of NCK1 in NIH/3T3 cells decreases DNA synthesis stimulated by EGF. Furthermore, the SH2 domain of NCK2 inhibits EGF- and PDGF-induced DNA synthesis. The SH3 domain of NCK binds a proline-rich domain on PAK, a known actin cytoskeleton regulator. The NCK protein thus mediates the interaction between PAK and RAC. The NCK2 protein binds human PDGFR-b (Tyr-1009); overexpression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation. Various growth factor receptors, cell surface antigens and adhesion molecules phosphorylate mammalian NCK1 and NCK2.The human NCK1 and NCK2 genes map to chromosomes 3q21 and 2q12, respectively.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of NCK2 (I364) pAb in paraffin-embedded human colon carcinoma tissue.

Bioworld Molecular Weight: ~ 43 kDa

Specificity: NCK2 (I364) polyclonal antibody detects endogenous levels of NCK2 protein.

Alternative Name: Cytoplasmic protein NCK2; Growth factor receptor-bound protein 4; NCK adaptor protein 2; Nck-2; SH2/SH3 adaptor protein NCK-beta; NCK2; GRB4

Immunogen: Synthetic peptide, corresponding to amino acids 341-390 of Human Nck-2.

Conjugate: Unconjugated

Modification: Unmodified