Product Description

NCoA-3 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4237-50, ES4237-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein. [provided by RefSeq, Mar 2010],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: NCOA3, AIB1, BHLHE42, RAC3, TRAM1, Nuclear receptor coactivator 3, NCoA-3, ACTR, Amplified in breast cancer 1 protein, AIB-1, CBP-interacting protein, pCIP, Class E basic helix-loop-helix protein 42, bHLHe42, Receptor-associated coactivator 3, RAC-3, Steroid receptor coactivator protein 3, SRC-3, Thyroid hormone receptor activator molecule 1, TRAM-1

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from the Internal region of human NCoA-3.

Isotype: IgG

Target: NCoA-3

Specificity: NCoA-3 Polyclonal Antibody detects endogenous levels of NCoA-3 protein.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Widely expressed, High expression in heart, skeletal muscle, pancreas and placenta, Low expression in brain, and very low in lung, liver and kidney,

Observed Band (KD): 160kD

Fields: Endocrine resistance, Estrogen signaling pathway, Thyroid hormone signaling pathway, Pathways in cancer, Breast cancer

Function: Alternative Products: Additional isoforms seem to exist,Catalytic Activity: Acetyl-CoA + histone = CoA + acetylhistone
Domain: Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain)
Enzyme Regulation: Coactivator activity on nuclear receptors and NF-kappa-B pathways is enhanced by various hormones, and the TNF cytokine, respectively. TNF stimulation probably enhances phosphorylation, which in turn activates coactivator function. In contrast, acetylation by CREBBP apparently suppresses coactivation of target genes by disrupting its association with nuclear receptors
Function: Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubuni

Subcellular Location: Cytoplasm Nucleus Mainly cytoplasmic and weakly nuclear Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus

Storage and Stability: -20°C/1 year

Gene Name: NCOA3

Protein Name: Nuclear receptor coactivator 3

Mouse SwissProt Number: O09000

Rat SwissProt Number: Q9EPU2

Research Use Only