Product Description

NEDD4L (phospho-Ser342) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES14530-50, ES14530-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: E3 ubiquitin-protein ligase NEDD4-like (EC 6.3.2.-) (NEDD4.2) (Nedd4-2)

Clonality: Polyclonal

Immunogen: Synthesized phosho peptide around human NEDD4L (Ser342)

Isotype: IgG

Target: NEDD4L

Specificity: This antibody detects endogenous levels of Human Mouse Rat NEDD4L (phospho-Ser342)

Dilution: WB 1:1000-2000

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Ubiquitously expressed, with highest levels in prostate, pancreas, and kidney (PubMed:14615060, PubMed:15496141, PubMed:19664597), Expressed in melanocytes (PubMed:23999003),

Observed Band (KD): 110-120kD

Fields: Ubiquitin mediated proteolysis, Endocytosis, Tight junction, Aldosterone-regulated sodium reabsorption

Function: Function: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK
Induction: By androgens in prostate, and by albumin in kidney
Pathway: Protein modification, protein ubiquitination
PTM: Phosphorylated by SGK or PKA, which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation
Similarity: Contains 1 C2 domain
Similarity: Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain
Similarity: Contains 4 WW domains
Subunit: Int

Subcellular Location: Cytoplasm Golgi apparatus Endosome, multivesicular body May be recruited to exosomes by NDFIP1

Storage and Stability: -20°C/1 year

Gene Name: NEDD4L KIAA0439 NEDL3

Protein Name: NEDD4L (Ser342)

Mouse SwissProt Number: Q8CFI0

Research Use Only