Nestin Polyclonal Antibody-BS71485

Nestin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS71485-50, BS71485-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P48681

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:1000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:100

Background: This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 150KDa/177KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of Neuro-2a cells, using Nestin antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s., Immunohistochemistry of paraffin-embedded Mouse brain using Nestin antibody at dilution of 100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded Rat brain using Nestin antibody at dilution of 100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunofluorescence analysis of Neuro-2a cells using Nestin Rabbit pAb at dilution of 1:50. Blue: DAPI for nuclear staining.

Alternative Name: NES; Nbla00170; nestin; Nestin

Immunogen: A synthetic peptide of human NES(NP_006608.1).

Conjugate: Unconjugated

Modification: Unmodified