Product Description

Neu (phospho Tyr1112) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES5142-50, ES5142-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding d

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: ERBB2, HER2, MLN19, NEU, NGL, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, CD antigen CD340

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human HER2 around the phosphorylation site of Tyr1112. AA range:1081-1130

Isotype: IgG

Target: HER2

Specificity: Phospho-Neu (Y1112) Polyclonal Antibody detects endogenous levels of Neu protein only when phosphorylated at Y1112.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth,

Observed Band (KD): 180kD

Fields: EGFR tyrosine kinase inhibitor resistance, Endocrine resistance, Platinum drug resistance, MAPK signaling pathway, ErbB signaling pathway, Calcium signaling pathway, HIF-1 signaling pathway, PI3K-Akt signaling pathway, Focal adhesion, Adherens junction, Tight junction, Pathways in cancer, Proteoglycans in cancer, MicroRNAs in cancer, Pancreatic cancer, Endometrial cancer, Prostate cancer, Bladder cancer, Non-small cell lung cancer, Breast cancer, Gastric cancer, Central carbon metabolism in cancer

Function: Catalytic Activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Disease: Defects in ERBB2 are associated with familial glioma of brain [MIM: 137800], also called glioblastoma multiforme. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas
Disease: Defects in ERBB2 are associated with gastric cancer [MIM: 137215], also known as hereditary familial diffuse gastric cancer (HDGC)
Disease: Defects in ERBB2 are associated with lung cancer [MIM: 211980], also called adenocarcinoma of lung
Disease: Defects in ERBB2 are associated with ovarian cancer [MIM: 167000]. Ovarian cancer is the leading cause of death from gynecologic malignancy. It is characterized by advanced presentation with loco-regional dissemination in the peritoneal cavity and the rare incidence of viscera

Subcellular Location: [Isoform 1]: Cell membrane, Single-pass type I membrane protein Early endosome Cytoplasm, perinuclear region Nucleus Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1 Also detected in VPS35-positive endosome-to-TGN retrograde vesicles (PubMed:31138794), [Isoform 2]: Cytoplasm Nucleus, [Isoform 3]: Cytoplasm Nucleus

Storage and Stability: -20°C/1 year

Gene Name: ERBB2

Protein Name: Receptor tyrosine-protein kinase erbB-2

Mouse Gene ID: 13866

Mouse SwissProt Number: P70424

Rat SwissProt Number: P06494

Research Use Only