Product Description

NFκB-p100 (phospho Ser872) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES6379-50, ES6379-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: nuclear factor kappa B subunit 2(NFKB2) Homo sapiens This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, ELISA

Alternate Names: NFKB2, LYT10, Nuclear factor NF-kappa-B p100 subunit, DNA-binding factor KBF2, H2TF1, Lymphocyte translocation chromosome 10 protein, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, Oncogene Lyt-10, Lyt10

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human NF-kappaB p100 around the phosphorylation site of Ser872. AA range:838-887

Isotype: IgG

Target: NF-κB p100/p52

Specificity: Phospho-NFκB-p100 (S872) Polyclonal Antibody detects endogenous levels of NFκB-p100 protein only when phosphorylated at S872.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Leukemia, Lymph, Thymus,

Observed Band (KD): 96kD

Fields: MAPK signaling pathway, NF-kappa B signaling pathway, Osteoclast differentiation, C-type lectin receptor signaling pathway, Legionellosis, Human T-cell leukemia virus 1 infection, Epstein-Barr virus infection, Pathways in cancer, Viral carcinogenesis, Breast cancer

Function: Disease: A chromosomal aberration involving NFKB2 is found in a case of B-cell non Hodgkin lymphoma (B-NHL). Translocation t(10, 14)(q24, q32) with IGHA1. The resulting oncogene is also called Lyt-10C alpha variant
Disease: A chromosomal aberration involving NFKB2 is found in a cutaneous T-cell leukemia (C-TCL) cell line. This rearrangement produces the p80HT gene which encodes for a truncated 80 kDa protein (p80HT)
Disease: In B-cell leukemia (B-CLL) cell line, LB40 and EB308, can be found after heterogeneous chromosomal aberrations, such as internal deletions
Domain: The C-terminus of p100 might be involved in cytoplasmic retention, inhibition of DNA-binding by p52 homodimers, and/or transcription activation
Domain: The glycine-rich region (GRR) appears to be a critical element in the generation of p52
Function: NF-kappa-B is a pleiotropic transcription factor which is present in almost a

Subcellular Location: Nucleus Cytoplasm Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B)

Storage and Stability: -20°C/1 year

Gene Name: NFKB2

Protein Name: Nuclear factor NF-kappa-B p100 subunit

Mouse Gene ID: 18034

Mouse SwissProt Number: Q9WTK5

Research Use Only