Product Description

NF-kappaB p105 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66576-50, MB66576-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses. There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regμlate transcription. In unstimμlated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regμlates gene expression. NIK and IKKα (IKK1) regμlate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus. Following IKK-mediated phosphorylation of p105 NF-κB at mμltiple sites (Ser921, 923, 927, and 932) on its carboxy-terminus, SCF/β-TrCP mediated processing produces the 50 kDa active form p50.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Mouse

Applications: WB

Alternate Names: Nuclear factor NF-kappa-B p105 subunit, DNA-binding factor KBF1, EBP-1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant human NF-κB1 p105/p50 protein.

Dilution: WB (1/500 - 1/1000)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of NF-kappaB p105 protein.

Molecular Weight: ~ 105 kDa

Pathway: Regμlation of Apoptosis，Death Receptor Signaling，Inhibition of Apoptosis，NF-kB Signaling

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P19838

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only