Product Description

NF-kappaB p65 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB65963-50, MB65963-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses. There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regμlate transcription. In unstimμlated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regμlates gene expression. NIK and IKKα (IKK1) regμlate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Mouse

Applications: WB, IHC, IF/ICC, IP

Alternate Names: NFKB3, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant protein corresponding to human NF-kappaB p65.

Dilution: WB (1/500 - 1/1000), IHC (1/200 - 1/500), IF/ICC (1/100 - 1/200), IP (1/100 - 1/200)

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of NF-kappaB p65 protein.

Molecular Weight: ~ 65 kDa

Pathway: Regμlation of Apoptosis, Death Receptor Signaling, Inhibition of Apoptosis, NF-kB Signaling, Signaling Pathways Activating p38 MAP Kinase, T cell receptor signaling, Toll-like Receptor signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q04206

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only