Product Description

Nibrin (P461) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2230-50, BS2230-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O60934

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex (MRN complex). This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions. In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells. NBS1 is expressed ubiquitously and presents high levels in testis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Nibrin (P461) polyclonal antibody detects endogenous levels of Nibrin protein.

Bioworld Molecular Weight: ~ 95 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Nibrin (P461) pAb at 1:1000 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: HepG2 whole cell lysate (40ug)
Lane 3: LO2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Nibrin (P461) pAb in paraffin-embedded lung carcinoma tissue at 1:50 showing Nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Nibrin (P461) pAb in paraffin-embedded lung carcinoma tissue at 1:50 showing Nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Nijmegen breakage syndrome protein 1; Cell cycle regulatory protein p95; NBS1/P95; NBS; NBS1; P95

Immunogen: Synthetic peptide, corresponding to amino acids 431-480 of Human Nibrin.

Conjugate: Unconjugated

Modification: Unmodified