Product Description

NIPP1 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES6707-50, ES6707-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: PPP1R8, ARD1, NIPP1, Nuclear inhibitor of protein phosphatase 1, NIPP-1, Protein phosphatase 1 regulatory inhibitor subunit 8

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human PPP1R8. AA range:196-245

Isotype: IgG

Target: NIPP1

Specificity: NIPP1 Polyclonal Antibody detects endogenous levels of NIPP1 protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas, Less abundant in kidney, The concentration and ratio between isoforms is cell-type dependent, Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney, Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells,

Observed Band (KD): 40kD

Function: Cofactor: Magnesium. Endoribonuclease function is magnesium-dependent
Domain: Has a basic N- and C-terminal and an acidic central domain
Function: Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation
Function: Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing
Miscellaneous: A synthetic peptide, NIPP-1(330-351), is able to inhibit PP-1. Phosphorylation of Tyr-335 reduces PP-1 inhibition, whereas phosphorylation

Subcellular Location: Nucleus Nucleus speckle Primarily, but not exclusively, nuclear, [Isoform Gamma]: Cytoplasm Found mainly in the cytoplasm

Storage and Stability: -20°C/1 year

Gene Name: PPP1R8

Protein Name: Nuclear inhibitor of protein phosphatase 1

Mouse Gene ID: 100336

Mouse SwissProt Number: Q8R3G1

Research Use Only