Product Description

Nkx-3.1 (L2) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1456-50, BS1456-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99801

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

All Applications: IHC: 1:50~1:200

Background: The homeobox gene Nkx-3.1 is the human homolog of Drosophila bagpipe, which, in conjunction with tinman, determines cell fate in the dorsal mesoderm. In mammalian species, Nkx-3.1 is predominantly expressed in prostate, and it regulates prostate development in response to sonic hedgehog (Shh) signaling by exerting growth-suppressive and differentiating effects on prostatic epithelium. Nkx-3.1 is also expressed at lower levels in other tissues, including the heart and gut, in a Shh independent manner, where it plays a role in regulating proliferation of glandular epithelium and in the formation of ducts in prostate and minor salivary glands. Nkx-3.1 preferentially binds the TAAGTA sequence, which has not been reported for any other NK class homeoprotein. The human Nkx-3.1 gene is located on chromosome 8q21, which frequently undergoes a loss of heterozygosity, and although Nkx-3.1 is not a tumor suppressor gene, it may be a useful marker for benign and malignant prostate epithelium.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of Nkx-3. 1 (L2) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 38 kDa

Specificity: NKX3.1 (L2) polyclonal antibody detects endogenous levels of NKX3.1 protein.

Alternative Name: Homeobox protein Nkx-3.1; Homeobox protein NK-3 homolog A; NKX3-1; NKX3.1; NKX3A

Immunogen: Synthetic peptide, corresponding to the N-terminual of Human Nkx-3.1.

Conjugate: Unconjugated

Modification: Unmodified