Product Description

NLRC4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80502-50, BS80502-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NPP4

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 110KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of Mouse large intestine, using NLRC4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s., Immunofluorescence analysis of THP-1 cells using NLRC4 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of rat lung cells using NLRC4 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of mouse lung cells using NLRC4 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: NLRC4; AIFEC; CARD12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR2.1; FCAS4; IPAF

Immunogen: Recombinant fusion protein of human NLRC4(NP_001186067.1).

Conjugate: Unconjugated

Modification: Unmodified