Product Description

NOS1 (Y846) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1821-50, BS1821-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P29475

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NOS1 (Y846) polyclonal antibody detects endogenous levels of NOS1 protein.

Bioworld Molecular Weight: ~ 160 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of NOS1 (Y846) pAb at 1:500 dilution
Lane 1: PC3 whole cell lysate (40ug)
Lane 2: A549 whole cell lysate (40ug)
Lane 3: U-87MG whole cell lysate (20ug)
Lane 4: C6 whole cell lysate (40ug)
Lane 5: BV2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of NOS1 (Y846) pAb in paraffin-embedded human lung carcinoma tissue at 1:5000. cytoplasmic and cell membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of NOS1 (Y846) pAb in paraffin-embedded human kidney carcinoma tissue at 1:5000. cytoplasmic and cell membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Nitric oxide synthase, brain; Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1; bNOS; NOS1

Immunogen: Synthetic peptide, corresponding to amino acids 815-862 of Human NOS1.

Conjugate: Unconjugated

Modification: Unmodified