Product Description

NOS3 (phospho-T494) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4636-50, BS4636-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS), inducible NOS (iNOS) or macrophage NOS (mNOS), and endothelial NOS (eNOS).eNOS is a calcium/calmodμlin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible mμltifunctional second messenger NO from L arginine through a series of reactions in which L citrμlline is an intermediate. eNOS plays an important role in controlling vascμlar tone, platelet aggregation, and cardiac myocyte function.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IHC

Alternate Names: Nitric oxide synthase, endothelial, Constitutive NOS, cNOS, EC-NOS, Endothelial NOS, eNOS, NOS type III, NOSIII, NOS3

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human NOS3 around the phosphorylation site of Threonine 494.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-NOS3 (T494) polyclonal antibody detects endogenous levels of NOS3 protein when phosphorylated at Thr494. (removal of the initiator methionine)

Molecular Weight: ~ 140 kDa

Pathway: Hypoxia Signaling, Tumor Angiogenesis,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P29474

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only