Product Description

NOX4 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES11921-50, ES11921-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from part region of human protein

Isotype: IgG

Target: NOX4,

Specificity: NOX4 Polyclonal Antibody detects endogenous levels of protein.

Dilution: WB 1:500-2000 ELISA 1:5000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Expression: Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.

Observed Band (KD): 63kD

Fields: AGE-RAGE signaling pathway in diabetic complications, Alcoholic liver disease, Alzheimer disease, Pathways of neurodegeneration - multiple diseases, Chemical carcinogenesis - reactive oxygen species

Function: Developmental Stage: Expressed in fetal kidney and fetal liver. enzyme regulation:Inhibited by plumbagin (By similarity). Activated by phorbol 12-myristate 13-acetate (PMA). Activated by insulin. Inhibited by diphenylene iodonium. Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. Isoform 3 is not functional. Isoform 4 displays an increased activity while isoform 5 and isoform 6 display reduced activity. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. induc

Subcellular Location: Endoplasmic reticulum membrane, Multi-pass membrane protein. Cell membrane, Multi-pass membrane protein Cell junction, focal adhesion May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus., [Isoform 4]: Nucleus Nucleus, nucleolus

Storage and Stability: -20°C/1 year

Gene Name: NOX4, RENOX

Protein Name: NADPH oxidase 4, EC 1.6.3.-, Kidney oxidase-1, KOX-1, Kidney superoxide-producing NADPH oxidase, Renal NAD(P H-oxidase

Human Gene ID: 50507

Human SwissProt Number: Q9NPH5

Mouse SwissProt Number: Q9JHI8

Rat SwissProt Number: Q924V1

Research Use Only