Product Description

NPM (phospho-T234) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS4656-50, BS4656-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P06748

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities. The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: p-NPM (T234) polyclonal antibody detects endogenous levels of NPM protein only when phosphorylated at Thr234.

Bioworld Molecular Weight: ~ 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p-NPM1 (T234) polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 cell lysate treated with colchicine(0.2µg/ML,24h)
Lane 2: Raw264.7 cell treated with colchicine(0.2µg/ML,24h)
Lane 3: PC12 cell lysate treated with colchicine(0.2µg/ML,24h), Immunohistochemistry (IHC) analyzes of p-NPM (T234) pAb in paraffin-embedded human kidney carcinoma tissue at 1:5000. nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of p-NPM (T234) pAb in paraffin-embedded human breast carcinoma tissue at 1:5000. Nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Nucleophosmin; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; NPM1; NPM

Immunogen: Synthetic phosphopeptide derived from human NPM around the phosphorylation site of Threonine 234.

Conjugate: Unconjugated

Modification: Phosphorylation