Product Description

Nrf2 (Acetyl Lys599) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1138-50, ES1138-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: NFE2L2; NRF2; Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor, erythroid derived 2, like 2

Clonality: Polyclonal

Immunogen: Synthesized acetyl-peptide derived from the C-terminal region of human Nrf2 around the acetylation site of K599.

Isotype: IgG

Target: Nrf2,

Specificity: Acetyl-Nrf2 (K599) Polyclonal Antibody detects endogenous levels of Nrf2 protein only when acetylation at K599.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.

Observed Band (KD): 75-100kD

Fields: Protein processing in endoplasmic reticulum, Parkinson disease, Pathways in cancer, Chemical carcinogenesis - reactive oxygen species, Hepatocellular carcinoma, Lipid and atherosclerosis, Fluid shear stress and atherosclerosis

Function: Domain: Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus. Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. PTM: Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus. Similarity: Belongs to the bZIP family. Similarity: Belongs to the bZIP family. CNC subfamily. Similarity: Contains 1 bZIP domain. Subcellular Location: Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electr

Subcellular Location: Cytoplasm, cytosol Nucleus Cytosolic under unstressed conditions: ubiquitinated and degraded by the BCR(KEAP1) E3 ubiquitin ligase complex (PubMed:15601839, PubMed:21196497). Translocates into the nucleus upon induction by electrophilic agents that inactivate the BCR(KEAP1) E3 ubiquitin ligase complex (PubMed:21196497).

Storage and Stability: -20°C/1 year

Gene Name: NFE2L2

Protein Name: Nuclear factor erythroid 2-related factor 2

Human Gene ID: 4780

Human SwissProt Number: Q16236

Mouse Gene ID: 18024

Mouse SwissProt Number: Q60795

Rat Gene ID: 83619

Rat SwissProt Number: O54968

Research Use Only